![]() Similarly, in the absence of deproteinization, some of the half-site product in panel F runs as a smear and is slightly under-represented. This quantitation slightly under-represents the abundance of the SSC in panel F because some of the complex runs as a smear. It prepares the ends of the DNA so that they can successfully bind to the target genomic DNA via 3’-processing, it creates a complex with the viral DNA that is capable of transporting it into the nucleus, and it. Gottlinger Family History Gottlinger Name Meaning Historically, surnames evolved as a way to sort people into groups - by occupation, place of origin, clan affiliation, patronage, parentage, adoption, and even physical characteristics (like red hair). ( E, F) show quantitation of the data in panels C and D, respectively. Retroviral integrase is one of the key enzymes needed to integrate viral DNA into a host cell’s genome for many retroviruses including HIV. The band just above the 6 kb marker in panel B, lane 1 and in panel C results from a half-site insertion of another donor DNA molecule into the concerted integration product. Reaction mixtures were incubated for the indicated time and subjected to electrophoresis with (C) or without (D) deproteinization. ( C, D) Kinetics of STC formation parallels formation of concerted integration product. The migration position of linear size markers is shown on the left. ![]() ( B) The products of an integration reaction were subjected to electrophoresis in an agarose gel after deproteinization (lane 1), after the addition of EDTA (lane 2), EDTA and 600 mM NaCl (lane 3), EDTA and 10 μg/ml heparin (lane 4) and EDTA and 0.1% NP-40 (lane 5). ( A) Schematic showing the expected integration products after deproteinization. Initially, the enzyme removes di- or trinucleotides from viral DNA ends to expose 3-hydroxyls attached to the invariant CA dinucleotides (3-processing reaction). Concerted integration products are stably associated with integrase. Retroviral integrase (IN) is responsible for two consecutive reactions, which lead to insertion of a viral DNA copy into a host cell chromosome. ![]()
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